1. Eur J Clin Microbiol Infect Dis. Jun;35(6) doi: /s Epub Mar Since influenza viruses can cause severe illness, timely diagnosis is important for an adequate intervention. The available rapid detection methods either lack. The Solana Influenza A+B Assay is a rapid qualitative in vitro diagnostic test for the detection and differentiation of influenza A and influenza B viral RNA in nasal .

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Direct detection of influenza virus antigen in nasopharyngeal specimens by direct enzyme immunoassay in comparison with quantitating virus shedding. It has the potential to lead to pandemics due to its dynamic nature and large host range. Viewpoints Point-of-care diagnostics is a growing and dynamic infleunza helping to improve clinical and economic outcomes globally.

In order to correct for false-positive results, we obtained samples not only from symptomatic patients but also from asymptomatic individuals during the same influenza season. How are they similar? Efficacy and safety of the neuraminidase inhibitor zanamivir in influuenza treatment of influenzavirus infections.

The multiplex TaqMan PCR was more sensitive than standard conventional culturing or shell vial culturing; i. Please click here to return to the homepage. In fact, we were able to detect as little as 0. The samples from the outpatient clinic were sent to the laboratory within 2 h. Populations at high risk of complications include: Routine diagnostic logistics were used for sample transportation from the general practices to the laboratory as well as for sample transportation from the outpatient clinic to the laboratory.


Since experts must select the viruses to include in the vaccine months before flu season, there may not be a good match between the vaccine and the circulating viruses. Combined nose and throat swab specimens or nasal washes were obtained from individuals with upper or lower respiratory tract symptoms. Here are the instructions how to enable JavaScript in your web browser. Types of flu virus.

Lastly, be sure to get lots of rest and drink plenty of fluids to help your body fight the virus. What are the different types of influenza viruses? The CDC estimates that each year overall, influenza infection results in between 9. The available rapid detection methods either lack sensitivity or require complex laboratory manipulation. The applicability of this multiplex PCR was evaluated with 27 influenza virus A and 9 influenza virus B reference strains and isolates.

Antiviral medications are most effective when started within the first 48 hours of your illness. Results can be obtained within a few hours, thus allowing time for adequate clinical management and the evaluation of antiviral therapy. Influenza D is found mainly in cattle.

For example, the most common influenza A subtypes that circulate seasonally in humans are H1N1 and H3N2. Discover our Youtube channel. Schuurmanand A. Data for the to influenza season indicates that Add to your selection Do you need more information.

After absorption for 1 h at room temperature, the inoculum was removed and 5 ml of fresh Eagle minimal essential medium supplemented with 0. Getting Straight to the Point! Infectious Disease Our products and services include tests to assist in the diagnosis and management of many infectious diseases.

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Abstract Since influenza viruses can cause severe illness, timely diagnosis is important for an adequate intervention. Learn the Side Effects. The viruses selected for the vaccine are based off of research into which strains will be most common.

Simultaneous Detection of Influenza Viruses A and B Using Real-Time Quantitative PCR

How are influenza A and influenza B different? These subtypes are based off of the combination of two proteins on the viral surface: During flu season, having a scratchy throat, body aches, or fatigue can signal the arrival of the flu virus. For example, although virus isolation via cell culturing can be a robust and sensitive method for the detection of limited numbers of viable virions, it is labor-intensive and depends on optimal sample transport for sensitive virus isolation. For full functionality of this site it is necessary to enable JavaScript.

INFLUENZA A & B – Science

They can circulate simultaneously, but usually one type is dominant during a seasonal epidemic. This level of sensitivity correlated with 0.

Moreover, primers and probes were tested for possible interactions to make sure that they could be used together in a multiplex assay.