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More about how we get our images. Aspirate media from cultures; wash cells with 1X PBS; aspirate. Immediately scrape the cells off the plate and transfer the extract to a microcentrifuge tube. Prepare solutions with reverse osmosis deionized RODI or equivalent grade water.
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human FXR2 protein. Microcentrifuge for 5 min. Find answers on our FAQs page.
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From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Biotinylated Protein Ladder Detection Pack: Wash three times for 5 min each with 15 ml of TBST. Blotting Membrane and Paper: Fragile X syndrome is a genetic disorder characterized by a spectrum of physical and behavioral features and is a frequent form of inherited mental retardation 1. This can be done by re-exposing the blot to ECL reagents and making sure there is no signal prior to adding the next primary antibody.
Please refer to primary antibody datasheet or product webpage for recommended antibody dilution. Detection of Proteins Directions for Use: It should be noted that for the best possible results a fresh blot is always recommended.
Changing to another country might result in datashfet of shopping cart. Each of the fragile X proteins can self-associate, as well as form heteromers with the other two related proteins daasheet. Protein Blotting A general protocol for sample preparation. Volumes are for 10 cm x 10 cm cm 2 of membrane; for different sized membranes, adjust volumes accordingly. Dilute to 1X with dH 2 O.
FXR2 Antibody – Would you like to visit your country specific website? Incubate membrane in 25 ml of blocking buffer for 1 hr at room temperature.
Solutions and Reagents From sample preparation to detection, the reagents you need for your Western Blot are now in one convenient kit: Proceed with detection Section D. Application Dilutions Western Blotting 1: Additionally, it is recommended that you verify the removal of the first antibody complex prior to reprobing 5116 that signal attributed to binding of the new antibody is not leftover signal from the first immunoblotting experiment.
Polyclonal Antibody – FXR2 Antibody – Western Blotting, UniProt ID P51116, Entrez ID 9513 #4247
To Purchase S Dataxheet sizes. Reprobing of an existing membrane is a convenient means to immunoblot for multiple proteins independently when only a limited amount of sample is available.
Primary Antibody Dilution Buffer: These results dxtasheet that fragile X syndrome is related to abnormal translation caused by defects in RNAi-related pathways. Treat cells by adding fresh media containing regulator for desired time. Electrotransfer to nitrocellulose membrane Incubate substrate with membrane for 1 minute, remove excess solution membrane remains wetwrap in plastic and expose to X-ray film.
Prepare solutions with reverse osmosis deionized RODI or equivalently purified water. Reprobing can be a valuable method but with each reprobing of a blot there is potential for increased background signal. Antibodies are purified by protein A and peptide affinity chromatography. Do not aliquot the antibody. Sonicate for 10—15 sec to complete cell lysis and shear DNA to reduce sample viscosity.